Since the release of first label free biosensor -Biacore – in early 1990s the label free biosensors have become gold standards for measuring biomolecular interactions. Before the introduction of label free biosensing technology the predominant means for detection biomolecular interaction was based on ELISA/FIA format. Though widely used, these methods required binding partners to be labeled with enzyme, fluorophore or radiolabel for detection. Labels invariably affected the binding kinetics of interaction by modifying the structure of binding partners. In addition, the measurements were typically end point assay making it difficult to get association and dissociation constants of the binding reaction. Introduction of Biacore fundamentally altered the way the biomolecular interactions are studied by enabling measurement of binding event in real time. Since the introduction of Biacore, several other label free biosensing platforms have been introduced in the market. Most of them are based on optical properties like surface Plasmon resonance, diffraction gratings and Total internal Reflection. However, new technologies are being introduced into the market on a regular basis. For example measuring heat released or absorbed during binding event to measure kinetics or using micro-cantilever technology based on Atomic Force Microscopy platform to measure change in height. Below you will find a list of companies offering Label Free Biosensors.
Please let me know if I have missed some companies. Please acknowledge the site if you are disseminating this information anywhere else.
The list of companies has been updated with clickable links and is available in Resources page (Dec 28; 2009): Updated List of Label Free Biosensing Companies